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Title: | A cold-adapted endo-fucoidanase Psf1 from Pseudoalteromonas sp. that catalyzes production of T-cell activating fucoidan oligosaccharides from Saccharina latissima fucoidan, |
Authors: | Vo, Thi Dieu Trang Mikkelsen, Maria Dalgaard Monica Daugbjerg, Christensen Sebastian, Meier Cameron James, Hunt Holck, Jesper Hreggviðsson, Guðmundur Oli ´ Jona, Freysdottir Cao, Thi Thuy Hang Huynh, Hoang Nhu Khanh Meyer, Anne S. |
Keywords: | Brown seaweed Bioactive sulfated fucoidan Saccharina latissima |
Issue Date: | 2025 |
Series/Report no.: | International Journal of Biological Macromolecules, Volume 320, Part 3, 16 pp, 2025;https://doi.org/10.1016/j.ijbiomac.2025.145930 |
Abstract: | Bioactive sulfated fucoidans have high fucose content and are derived from brown seaweeds. Here we report the
discovery of the first cold-adapted endo-α(1 → 3)-fucoidanase (EC 3.2.1.211), Psf1. The psf1 gene was found in
the genome of Pseudoalteromonas sp. S3178, a bacterium isolated from a shrimp near Antarctica. Phylogenetic
analyses designated Psf1 as a putative member of glycoside hydrolase family 107 (GH107). Substrate selectivity
analysis confirmed Psf1 as being endo-acting and indicated that the enzyme catalyzes hydrolysis of α(1 → 3)-
glycosidic fucoidan linkages. Psf1 had temperature optimum of 10–30 ◦C but retained activity at 1 ◦C. Structural
modeling indicated similarity to the crystal structure of P5A_FcnA (Psychromonas sp. SW5A), yet distinct high
variability regions were identified by RMSF. Psf1 released low molecular weight fucoidan oligosaccharides from
Saccharina latissima fucoidan that dose-dependently reduced IL-12p40 secretion in dendritic cells, and lowered
IFN-γ and IL-10 levels in dendritic cells co-cultured with allogeneic CD4+ T-cells, without affecting IL-17
secretion, indicating a suppression of Th1-mediated immune response. Treatment of dendritic cells with
native fucoidan from S. latissima did not affect cell viability or cytokine secretion. These findings have potential
to enable new enzyme-assisted production, at low temperatures, of bioactive fucoidan oligosaccharides from
S. latissima grown in the Northern Hemisphere |
URI: | http://tvhdh.vnio.org.vn:8080/xmlui/handle/123456789/21520 |
ISSN: | 01418130 |
Appears in Collections: | Công bố khoa học ở tạp chí quốc tế - International research papers (Bibliographic record and/or full-text)
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