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Please use this identifier to cite or link to this item: http://tvhdh.vnio.org.vn:8080/xmlui/handle/123456789/21520

Title: A cold-adapted endo-fucoidanase Psf1 from Pseudoalteromonas sp. that catalyzes production of T-cell activating fucoidan oligosaccharides from Saccharina latissima fucoidan,
Authors: Vo, Thi Dieu Trang
Mikkelsen, Maria Dalgaard
Monica Daugbjerg, Christensen
Sebastian, Meier
Cameron James, Hunt
Holck, Jesper
Hreggviðsson, Guðmundur Oli ´
Jona, Freysdottir
Cao, Thi Thuy Hang
Huynh, Hoang Nhu Khanh
Meyer, Anne S.
Keywords: Brown seaweed
Bioactive sulfated fucoidan
Saccharina latissima
Issue Date: 2025
Series/Report no.: International Journal of Biological Macromolecules, Volume 320, Part 3, 16 pp, 2025;https://doi.org/10.1016/j.ijbiomac.2025.145930
Abstract: Bioactive sulfated fucoidans have high fucose content and are derived from brown seaweeds. Here we report the discovery of the first cold-adapted endo-α(1 → 3)-fucoidanase (EC 3.2.1.211), Psf1. The psf1 gene was found in the genome of Pseudoalteromonas sp. S3178, a bacterium isolated from a shrimp near Antarctica. Phylogenetic analyses designated Psf1 as a putative member of glycoside hydrolase family 107 (GH107). Substrate selectivity analysis confirmed Psf1 as being endo-acting and indicated that the enzyme catalyzes hydrolysis of α(1 → 3)- glycosidic fucoidan linkages. Psf1 had temperature optimum of 10–30 ◦C but retained activity at 1 ◦C. Structural modeling indicated similarity to the crystal structure of P5A_FcnA (Psychromonas sp. SW5A), yet distinct high variability regions were identified by RMSF. Psf1 released low molecular weight fucoidan oligosaccharides from Saccharina latissima fucoidan that dose-dependently reduced IL-12p40 secretion in dendritic cells, and lowered IFN-γ and IL-10 levels in dendritic cells co-cultured with allogeneic CD4+ T-cells, without affecting IL-17 secretion, indicating a suppression of Th1-mediated immune response. Treatment of dendritic cells with native fucoidan from S. latissima did not affect cell viability or cytokine secretion. These findings have potential to enable new enzyme-assisted production, at low temperatures, of bioactive fucoidan oligosaccharides from S. latissima grown in the Northern Hemisphere
URI: http://tvhdh.vnio.org.vn:8080/xmlui/handle/123456789/21520
ISSN: 01418130
Appears in Collections:Công bố khoa học ở tạp chí quốc tế - International research papers (Bibliographic record and/or full-text)

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