Abstract:
Chemical investigation of a marine-derived fungus Aspergillus sp. OPR23-FS01 resulted in the isolation of a new
unsaturated fatty acid featuring a terminal olefin, 10-methoxy-2-methylene-10-oxodecanoic acid (1), two new
natural compounds, 2-carboxy-2′-methyl-azobenzene (2) and 1,3,9-trimethyluric acid (3), and 11 previously
described metabolites, pantheric acid C (4), pantheric acid D (5), 2-furoic acid (6), 5-(hydroxymethyl)-2-furancarboxylic acid (7), sporogen-AO 1 (8), gliocladic acid (9), hydroheptelidic acid (10), ditryptophenaline (11),
emodin (12), uridine (13), and thymidine (14). Their structures were identified by comprehensive analyses of
the spectroscopic methods, including NMR and HRESI mass spectra. Compound 2 possesses a rare diazenyl group
among the previously reported fungal metabolites. Compound 8 showed significant cytotoxicity against both
HepG2 and MCF-7 cell lines in a dose-dependent manner, with IC50 values of 4.66 ± 0.24 and 5.54 ± 0.28 μM,
respectively. Compounds 1¡5 and 8¡11 displayed antimicrobial effect against Enterococcus faecalis
ATCC299212, with MIC values ranging from 32 to 128 μM, whereas 1, 4, 5, and 8¡11 inhibited Candida albicans
ATCC10231 growth, with MIC values in a range of 32–256 μM.