Chemical Composition, Structural Properties, and Bioactivity of Carrageenan from Field-Cultivated Betaphycus gelatinus

Abstract

This study investigates seasonal biomass variations in Betaphycus gelatinus, a red alga cultivated in the field in Ninh Thuan, Vietnam, along with the chemical composition, structural properties, and bioactivity of its carrageenan. Monthly measurements over a one-year period revealed peak growth (2.02% per day) and carrageenan yield (59.61%) in June, identifying it as the optimal harvest period. FTIR and NMR analyses of carrageenan extracted from field-cultivated B. gelatinus showed hybrid κ- and β-carrageenan forms and a unique pyruvylated β-carrageenan structure not previously reported for this species. Bioactivity assays indicated high antioxidant potential, with a total antioxidant capacity equivalent to 48.30 mg ascorbic acid/g carrageenan and an ABTS radical scavenging IC50 of 3.64 µg/mL. Additionally, antibacterial tests demonstrated strong inhibition of Listeria monocytogenes (12.00 mm inhibition zone). These findings suggest that field cultivation is a sustainable approach for carrageenan production, yielding bioactive compounds with promising applications in pharmaceuticals, cosmetics, and food preservation as a viable alternative to wild harvesting.