Effects of IAA and KIN on somatic embryogenesis and the effect of acadian marine plant extract powder content on micropropagule regeneration of the seaweed Kappaphycus striatus.

Abstract

The objective of this study was to perfect the callus culture method for propagatingKappaphycus striatus.Our research focuses on enhancing somatic embryo (SEs) induction from the callus and micropropaguleregeneration in SEs. In order to enhance the induction rate of SEs, the 16-week-old callus was transplantedinto a semi-solidified PES (0.4% agar) medium supplemented with 0.5–3.0 mg.L-1 3-indoleacetic acid (IAA) and 0.5–3.0 mg.L-1 kinetin (KIN), either alone or in combination. The results showed that the transplantationof the callus into a PES medium containing IAA and KIN (0.5–3.0 mg.L-1), both individually and in combination, significantly impacted the somatic embryogenesis of K. striatus. After 8 weeks of cultivation, the treatment with 1 mg.L-1 IAA + 2 mg.L-1 KIN exhibited the highest embryo induction rate (62.22 ± 5.09%)and the greatest number of SEs (182.00 ± 10.58 embryos/explant) compared to other treatments.Moreover, during the micropropagule regeneration stage, the commercial powdered extract of Ascophyllum nodosum(Acadian Marine Plant Extract Powder, AMPEP) at concentrations ranging from 5 ppm to 50 ppmwas found to enhance the rate of micropropagule regeneration from SEs of K. striatus. The optimalregeneration of SEs of K. striatus into complete micropropagules occurred in an AMPEP medium at a concentration of 20 ppm. Micropropagules cultured in this medium displayed the highest survival rate(75.6 ± 5.09%), number of branches (7.67 ± 0.58 branches/micropropagule), branch length (24.7 ± 1.53; 25 ± 1.00 mm), and fresh weight (173.7 ± 8.14 mg) compared to alternative treatments.